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Genetic diversity of Fusarium oxysporum f. sp. dianthi in Southern Spain

Castaño, Raúl and Scherm, Barbara and Avilés, Manuel (2014) Genetic diversity of Fusarium oxysporum f. sp. dianthi in Southern Spain. Journal of mycology, Vol. 2014 , Article ID 582672. ISSN 2356-7481. eISSN 2314-6168. Article.

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DOI: 10.1155/2014/582672


The diversity of races and prevalence of pathogenic populations of Fusarium oxysporum f. sp. dianthi (Fod) were surveyed in an area in southern Spain. From 54 farms, 132 isolates were collected from wilted carnation plants. Isolates were characterized by RAPDPCR, DNA sequence analysis of the TEF1-α gene, and race-specific molecular markers. Selected isolates from RAPD groups were phenotypically evaluated by pathogenicity tests. Data analysis showed that Fod race 2 was the most frequent and prevalent race in the study area, followed by race 1/8. Moreover, phylogenetic analyses showed similar results, which were different to those of the race-specific PCR assays. It was concluded that (i) seven isolates were not classified in groups where Fod testers were clustered; even they showed different results when race-specific markers were used, (ii) ten isolates with retarded race 1 or race 8 specific band were characterized as F. proliferatum by TEF1-α gene sequencing and clustered into an outgroup, and (iii) six isolates failed to generate an amplification signal using race-specific markers. Furthermore, three of them were grouped close to race 2 tester according to the phylogenetic analyses, showing the same differential pathogenicity as race 2. This may indicate a Fod race 2 subgroup in this region.

Item Type:Article
ID Code:9966
Uncontrolled Keywords:Fusarium oxysporum f. sp. dianthi, TEF1-α gene, Spain
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
Publisher:Hindawi Publishing Corporation
Copyright Holders:© 2014 Raúl Castaño et al.
Deposited On:15 Jul 2014 09:11

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