titoli, abstracts, parole chiave >>>
PCR primers based on different portions of insertion elements can assist genetic relatedness studies, strain fingerprinting and species identification in rhizobia

Muresu, Rosella and Sulas, Leonardo and Polone, Elisa and Squartini, Andrea (2005) PCR primers based on different portions of insertion elements can assist genetic relatedness studies, strain fingerprinting and species identification in rhizobia. FEMS Microbiology Ecology, Vol. 54 (3), p. 445-453. eISSN 1574-6941. Article.

Full text not available from this repository.

DOI: 10.1016/j.femsec.2005.05.008

Abstract

Using the sequence of an insertion element originally found in Rhizobium sullae, the nitrogen-fixing bacterial symbiont of the legume Hedysarum coronarium, we devised three primer pairs (inbound, outbound and internal primers) for the following applications: (a) tracing genetic relatedness within rhizobia using a method independent of ribosomal inheritance, based on the presence and conservation of IS elements; (b) achieve sensitive and reproducible bacterial fingerprinting; (c) enable a fast and unambiguous detection of rhizobia at the species level. In terms of taxonomy, while in line with part of the 16S rRNA gene- and glutamine synthetase I-based clustering, the tools appeared nonetheless more coherent with the actual geographical ranges of origin of rhizobial species, strengthening the European–Mediterranean connections and discerning them from the asian and american taxa. The fingerprinting performance of the outward-pointing primers, designed upon the inverted repeats, was shown to be at least as sensitive as BOX PCR, and to be functional on a universal basis with all 13 bacterial species tested. The primers designed on the internal part of the transposase gene instead proved highly species-specific for R. sullae, enabling selective distinction from its most related species, and testing positive on every R. sullae strain examined, fulfilling the need of PCR-mediated species identification. A general use of other IS elements for a combined approach to rhizobial taxonomy and ecology is proposed.

Item Type:Article
ID Code:9952
Status:Published
Refereed:Yes
Uncontrolled Keywords:Rhizobium, IS element, strain fingerprinting, Rhizobial genetic diversity
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto per il sistema produzione animale in ambiente Mediterraneo, Sassari
Publisher:Blackwell / Wiley
eISSN:1574-6941
Copyright Holders:© 2005 Federation of European Microbiological Societies
Deposited On:07 Jul 2014 09:41

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page