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Antigenic epitopes of MAP2694 homologous to T-cell receptor gamma-chain are highly recognized in multiple sclerosis Sardinian patients

Cossu, Davide and Masala, Speranza and Frau, Jessica and Mameli, Giuseppe and Marrosu, Maria Giovanna and Cocco, Eleonora and Sechi, Leonardo Antonio (2014) Antigenic epitopes of MAP2694 homologous to T-cell receptor gamma-chain are highly recognized in multiple sclerosis Sardinian patients. Molecular Immunology, Vol. 57 (2), p. 138-140. ISSN 0161-5890. eISSN 1872-9142. Article.

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DOI: 10.1016/j.molimm.2013.09.001

Abstract

Mycobacterium avium ss. paratuberculosis (MAP) is an intracellular pathogen recently associated with multiple sclerosis (MS). Aiming to identify immunodominant epitopes belonging to MS related protein MAP2694 (UniProt accession no. Q73WG6), we investigated the binding activity of selected peptides against MS Sardinian sera. An overlapping 9-mers peptide library was synthesized spanning the entire aminoacidic sequence of the protein. Peripheral blood was collected from 47 MS patients and 42 sex and age matched healthy volunteers and subjected to enzyme-linked immunosorbent assay (ELISA) in order to investigate the reaction against the linear peptides generated. Two out of 58 synthetic 9-mers were strongly recognized by MS patients’ antibodies compared to controls. A competitive inhibition assay demonstrated that these two epitopes are immunodominant antibody targets within MAP2694 protein, as sera pre-adsorbed with these peptides were able to significantly block the antibody reaction to the MAP2694 protein, even if at a lesser extent than MAP2694 protein itself.

Item Type:Article
ID Code:9825
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium avium ss. paratuberculosis, Multiple sclerosis, molecular mimicry, ELISA, peptide library
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Elsevier
ISSN:0161-5890
eISSN:1872-9142
Deposited On:23 May 2014 12:34

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