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A Novel method for the determination of ascorbic acid and antioxidant capacity in Opuntia ficus indica using in vivo microdialysis

Pretti, Luca and Bazzu, Gianfranco and Serra, Pier Andrea and Nieddu, Giovanni (2014) A Novel method for the determination of ascorbic acid and antioxidant capacity in Opuntia ficus indica using in vivo microdialysis. Food chemistry, Vol. 147 , p. 131-137. ISSN 0308-8146. eISSN 1873-7072. Article.

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DOI: 10.1016/j.foodchem.2013.09.120

Abstract

A simple and rapid method was developed for in vivo simultaneous determination of ascorbic-acid and antioxidant capacity in microdialysates from cladodes of Opuntia ficus-indica (L.) Miller. The method is verified in water-stressed plants, as compared with a well-watered test controls. The microdialysis probe construction and insertion procedure was specifically developed to minimise the tissue trauma of the plant and to obtain optimal dialysis performance. Microdialysis was performed using a flow rate of 3 μL/min and the samples were analysed by HPLC coupled to electrochemical detection of ascorbic-acid and DPPH-determined antioxidant capacity. Our data indicate exponential decay of the concentrations of the analysed compounds as a function of microdialysis sampling time. Water-stressed Opuntia show decreased ascorbic acid levels and increased the others antioxidants.

Item Type:Article
ID Code:9808
Status:Published
Refereed:Yes
Uncontrolled Keywords:Microdialysis, ascorbic acid, vitamin C equivalent antioxidant capacity (VCEAC), antioxidant capacity, DPPH, Cactus pear
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 07 - Scienze agrarie e veterinarie > AGR/03 Arboricoltura generale e coltivazioni arboree
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:Elsevier
ISSN:0308-8146
eISSN:1873-7072
Copyright Holders:© 2013 Elsevier
Deposited On:19 May 2014 12:07

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