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Detection and quantification of Vibrio parahaemolyticus in shellfish from Italian production areas

Suffredini, Elisabetta and Mioni, Renzo and Mazzette, Rina and Bordin, Paola and Serratore, Patrizia and Fois, Federica and Piano, Annamaria and Cozzi, Loredana and Croci, Luciana (2014) Detection and quantification of Vibrio parahaemolyticus in shellfish from Italian production areas. International journal of food microbiology, Vol. 184 , p. 14-20. ISSN 0168-1605. eISSN 1879-3460. Article.

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DOI: 10.1016/j.ijfoodmicro.2014.04.016

Abstract

Vibrio parahaemolyticus is a marine microorganism, recognized as an important cause of foodborne illness particularly in Asia, South America and United States. Outbreaks are rarely reported in Europe, but they can occur unexpectedly in relation, among other reasons, to the spread of highly virulent strains. It is known that the risk is proportional to exposure levels to pathogenic V. parahaemolyticus (i.e. carrying the tdh and/or the trh genes) but currently there is a lack of occurrence data for pathogenic V. parahaemolyticus in shellfish production areas of the Member States. In this study a total of 147 samples of bivalve molluscs, from harvesting areas of two Italian regions (Sardinia and Veneto) were analyzed for Escherichia coli and salmonella, according to Reg 2073/2005, and for detection and enumeration of total and toxigenic V. parahaemolyticus strains using a new DNA colony hybridization method. Environmental parameters (water temperature and salinity) were also recorded. Results of E. coli were consistently in agreement with the legislation limits for the harvesting class of origin and Salmonella was detected only in one sample. The average contamination levels for total V. parahaemolyticus were 84 and 73 CFU/g respectively for Sardinia and Veneto, with the highest value reaching 8.7 × 103 CFU/g. Nineteen samples (12.9%) resulted positive for the presence of potentially pathogenic V. parahaemolyticus strains, with levels ranging between 10 and 120 CFU/g and most of the positive samples (n = 17) showing values equal or below 20 CFU/g. A significant correlation (r = 0.41) was found between water temperature and V. parahaemolyticus levels, as well as with isolation frequency.
The data provided in this study on contamination levels of total and potentially pathogenic V. parahaemolyticus, seasonal distribution and correlation with water temperature, will help in defining appropriate monitoring programs and post-harvest policies for this hazard, improving the management of the harvesting areas and the safety of bivalve molluscs.

Item Type:Article
ID Code:9799
Status:Published
Refereed:Yes
Uncontrolled Keywords:Vibrio parahaemolyticus, colony hybridization, quantitative analysis, tdh, trh, shellfish
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/04 Ispezione degli alimenti di origine animale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
ISSN:0168-1605
eISSN:1879-3460
Deposited On:19 May 2014 09:17

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