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Detection of genes encoding for virulence and adherence factors in Escherichia coli isolated in slaughtered Sarda breed sheep

Busia, Gianluca and Mureddu, Anna and Mazza, Roberta and Meloni, Domenico and Consolati, Simonetta Gianna and Piras, Francesca and Burgess, Catherine M. and Duffy, Geraldine and Mazzette, Rina (2014) Detection of genes encoding for virulence and adherence factors in Escherichia coli isolated in slaughtered Sarda breed sheep. Veterinary Microbiology, Vol. 168 (1), p. 234-239. ISSN 0378-1135. eISSN 1873-2542. Article.

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DOI: 10.1016/j.vetmic.2013.10.022

Abstract

In order to investigate the pathogenic profile of Escherichia coli hosted in “Sarda” sheep, autochthonous race present in Sardinia, thirty-seven E. coli strains collected from different sources (fleeces, carcass swabs and gut mucosa) of pre-chill slaughtered sheep (ewes and lambs) were serotyped using pheno- and genotypic methods. Furthermore, the presence of genes encoding for virulence factors and mediating for localized mucosal adherence factors was investigated, and pulsed-field gel electrophoresis (PFGE) characterization was performed.
Twenty-one (56.8%) of the isolates belonged to O91 serogroup and sixteen (43.2%) belonged to nine different serotypes (O5:H11, O8:H14, O26:H2, O38:H26, O116:H9, O116:H11, O132:H34, O149:H?, O161:H-). Of these non-O91 strains, five (13.5%) were able to produce verocytotoxin (VT) and were ascribed to VTEC pathogroup, eleven (29.7%) were attributed to the Enteropathogenic E. coli (EPEC) pathogroup; the other strains (n.21) cannot be ascribed to a pathogenic group. However, various associated virulence genes were observed in all isolated strains. Macrorestriction analysis highlighted a large heterogeneity of the E. coli strains.
The results confirm the role of sheep as reservoir of pathogenic E. coli serotypes potentially able to colonize and to damage the intestinal mucosa.

Item Type:Article
ID Code:9780
Status:Published
Refereed:Yes
Uncontrolled Keywords:Escherichia coli, sheep, serotyping, associated virulence factors, PCR
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/04 Ispezione degli alimenti di origine animale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
ISSN:0378-1135
eISSN:1873-2542
Deposited On:02 May 2014 12:54

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