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Dose-dependent effect of melatonin on postwarming development of vitrified ovine embryos

Succu, Sara and Pasciu, Valeria and Manca, Maria Elena and Chelucci, Sara and Torres Rovira, Laura and Leoni, Giovanni Giuseppe and Zinellu, Angelo and Carru, Ciriaco and Naitana, Salvatore and Berlinguer, Fiammetta (2014) Dose-dependent effect of melatonin on postwarming development of vitrified ovine embryos. Theriogenology, Vol. 81 (8), p. 1058-1066. ISSN 0093-691X. eISSN 1879-3231. Article.

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DOI: 10.1016/j.theriogenology.2014.01.032

Abstract

After cryopreservation, embryos become sensitive to the oxidative stress, resulting in lipid peroxidation, membrane injury, and structural destruction. The present study aimed to assess the effect of increasing concentration of melatonin during postwarming culture on embryo's ability to restore its functions after cryopreservation. In vitro–produced blastocysts were vitrified, warmed, and cultured in vitro in TCM 199 with 5 different supplementations: control (CTR): 10% fetal calf serum; bovine serum albumin (BSA): 0.04% (wt/vol) BSA; and MEL−3, MEL−6, MEL−9: BSA plus melatonin 10−3, 10−6, and 10−9 M. The medium with the highest melatonin concentration had the highest trolox equivalent antioxidant capacity, whose values were comparable with those determined in plasma sampled from adult ewes (8.7 ± 2.4 mM). The other media had lower trolox equivalent antioxidant capacity values (P < 0.01), below the range of the plasma. At the same time, embryos cultured with the highest melatonin concentration reported a lower in vitro viability, as evaluated by lower re-expansion and hatching rates, and lower total cell number compared with the other groups (P < 0.05). Their metabolic status was also affected, as evidenced by higher oxidative and apoptotic index and lower ATP concentration. The beneficial effects of melatonin on embryo development during postwarming culture were observed only at low concentration (10−9 M). These results suggest that melatonin at high concentration may exert some degree of toxic activity on pre-implantation embryos. Thus, the dose at which the embryos are exposed is pivotal to obtain the desiderate effect.

Item Type:Article
ID Code:9760
Status:Published
Refereed:Yes
Uncontrolled Keywords:In vitro embryo production, oxidative stress, apoptosis, ATP, total antioxidant capacity
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
ISSN:0093-691X
eISSN:1879-3231
Deposited On:15 Apr 2014 10:42

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