titoli, abstracts, parole chiave >>>
LRRK2 affects vesicle trafficking, neurotransmitter extracellular level and membrane receptor localization

Migheli, Rossana and Del Giudice, Maria Grazia and Spissu, Ylenia and Sanna, Giovanna and Xiong, Yulan and Dawson, Ted M. and Dawson, Valina L. and Galioto, Manuela and Rocchitta, Gaia Giovanna Maria and Biosa, Alice and Serra, Pier Andrea and Carri, Maria Teresa and Crosio, Claudia and Iaccarino, Ciro (2013) LRRK2 affects vesicle trafficking, neurotransmitter extracellular level and membrane receptor localization. PLoS One, Vol. 8 (10), e77198. eISSN 1932-6203. Article.

[img]
Preview
Full text disponibile come PDF Richiede visualizzatore di PDF come GSview, Xpdf o Adobe Acrobat Reader
Available under License Creative Commons Attribution.

2212Kb

DOI: 10.1371/journal.pone.0077198

Abstract

The leucine-rich repeat kinase 2 (LRRK2) gene was found to play a role in the pathogenesis of both familial and sporadic Parkinson’s disease (PD). LRRK2 encodes a large multi-domain protein that is expressed in different tissues. To date, the physiological and pathological functions of LRRK2 are not clearly defined. In this study we have explored the role of LRRK2 in controlling vesicle trafficking in different cellular or animal models and using various readouts. In neuronal cells, the presence of LRRK2G2019S pathological mutant determines increased extracellular dopamine levels either under basal conditions or upon nicotine stimulation. Moreover, mutant LRRK2 affects the levels of dopamine receptor D1 on the membrane surface in neuronal cells or animal models. Ultrastructural analysis of PC12-derived cells expressing mutant LRRK2G2019S shows an altered intracellular vesicle distribution. Taken together, our results point to the key role of LRRK2 to control vesicle trafficking in neuronal cells.

Item Type:Article
ID Code:9667
Status:Published
Refereed:Yes
Uncontrolled Keywords:Leucine-rich repeat kinase 2 (LRRK2), Parkinson’s disease (PD), neuronal cells
Subjects:Area 05 - Scienze biologiche > BIO/11 Biologia molecolare
Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:Public Library of Science
eISSN:1932-6203
Copyright Holders:© 2013 Migheli et al.
Deposited On:10 Mar 2014 09:57

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page