Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis

Dimauro, Corrado and Bacciu, Nicola and Macciotta, Nicolò Pietro Paolo (2007) Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis. Italian Journal of Animal Science, Vol. 6 (Suppl. 1), p. 122-124. eISSN 1828-051X. Article.

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Abstract

Four different normalisation techniques were applied for the corrections of fluorescence data generated by a cDNA microarray experiment. Correction for inaccurate signals and possible bias induced by fluorescence intensity, background intensity and dye effect were used in different combinations. Results of the present study highlight a pronounced role for the normalisation techniques in the absolute number of genes different expressed and a low concordance between different methods. Moreover, a significant effect of the dependent variable used, mean or median fluorescence intensity, was observed.

Item Type:	Article
ID Code:	965
Status:	Published
Refereed:	Yes
Uncontrolled Keywords:	Normalisation techniques, cDNA, statistical test
Subjects:	Area 07 - Scienze agrarie e veterinarie > AGR/17 Zootecnica generale e miglioramento genetico Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:	001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
Publisher:	Avenue media on behalf of Scientific Association of Animal Production (ASPA)
eISSN:	1828-051X
Publisher Policy:	Depositato per gentile concessione dell'ASPA
Additional Information:	Relazione presentata al 17. Congresso nazionale ASPA, Alghero, 29 maggio-1 giugno 2007.
Deposited On:	18 Aug 2009 10:03

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