Dimauro, Corrado and Bacciu, Nicola and Macciotta, Nicolò Pietro Paolo (2007) Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis. Italian Journal of Animal Science, Vol. 6 (Suppl. 1), p. 122-124. eISSN 1828-051X. Article.
Four different normalisation techniques were applied for the corrections of fluorescence data generated by a cDNA microarray experiment. Correction for inaccurate signals and possible bias induced by fluorescence intensity, background intensity and dye effect were used in different combinations. Results of the present study highlight a pronounced role for the normalisation techniques in the absolute number of genes different expressed and a low concordance between different methods. Moreover, a significant effect of the dependent variable used, mean or median fluorescence intensity, was observed.
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