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Genetic and metabolite diversity of Sardinian populations of Helichrysum italicum

Melito, Sara and Sias, Angela and Petretto, Giacomo Luigi and Chessa, Mario and Pintore, Giorgio Antonio Mario and Porceddu, Andrea (2013) Genetic and metabolite diversity of Sardinian populations of Helichrysum italicum. PLoS One, Vol. 8 (11), e79043. eISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0079043

Abstract

Background: Helichrysum italicum (Asteraceae) is a small shrub endemic to the Mediterranean Basin, growing in fragmented and diverse habitats. The species has attracted attention due to its secondary metabolite content, but little effort has as yet been dedicated to assessing the genetic and metabolite diversity present in these populations. Here, we describe the diversity of 50 H. italicum populations collected from a range of habitats in Sardinia.
Methods: H. italicum plants were AFLP fingerprinted and the composition of their leaf essential oil characterized by GC-MS. The relationships between the genetic structure of the populations, soil, habitat and climatic variables and the essential oil chemotypes present were evaluated using Bayesian clustering, contingency analyses and AMOVA.
Key results: The Sardinian germplasm could be partitioned into two AFLP-based clades. Populations collected from the southwestern region constituted a homogeneous group which remained virtually intact even at high levels of K. The second, much larger clade was more diverse. A positive correlation between genetic diversity and elevation suggested the action of natural purifying selection. Four main classes of compounds were identified among the essential oils, namely monoterpenes, oxygenated monoterpenes, sesquiterpenes and oxygenated sesquiterpenes. Oxygenated monoterpene levels were significantly correlated with the AFLP-based clade structure, suggesting a correspondence between gene pool and chemical diversity.
Conclusions: The results suggest an association between chemotype, genetic diversity and collection location which is relevant for the planning of future collections aimed at identifying valuable sources of essential oil.

Item Type:Article
ID Code:9633
Status:Published
Refereed:Yes
Uncontrolled Keywords:Helichrysum italicum, Asteraceae, Sardinia
Subjects:Area 05 - Scienze biologiche > BIO/15 Biologia farmaceutica
Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 02 Centri > Centro per la conservazione e valorizzazione della biodiversità vegetale
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
Publisher:Public Library of Science
eISSN:1932-6203
Copyright Holders:© 2013 Melito et al.
Deposited On:20 Feb 2014 11:49

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