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An Innovative and high-speed technology for sweater monitoring of Asinara Gulf (Sardinia- Italy)

Sighicelli, Maria and Iocola, Ileana and Pittalis, Daniele and Lugliè, Antonella Gesuina Laura and Padedda, Bachisio Mario and Pulina, Silvia and Iannetta, Massimo and Menicucci, Ivano and Fiorani, Luca and Palucci, Antonio (2014) An Innovative and high-speed technology for sweater monitoring of Asinara Gulf (Sardinia- Italy). Open Journal of Marine Science, Vol. 4 , p. 31-41. ISSN 2161-7384. eISSN 2161-7392. Article.

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DOI: 10.4236/ojms.2014.41005

Abstract

Laser induced fluorescenze technique for sea water monitoring allows no-time consuming, non-invasive and non-destructive controls. In this study, the performance of the new shipboard laser spectrofluorometric CAS-PER (Compact and Advanced Laser Spectrometer –ENEA Patent) for monitoring phytoplankton community composition was examined. The prototype CASPER is based on double laser excitation of water samples in the UV (266 nm) and visible (405 nm) spectral region and a double water filtration in order to detect both quantitative data, such as choromophoric dissolved organic matter (CDOM), proteins-like components (tyrosine, tryptophan), algal pigments (chlorophylls a and b, phycoerythrin, phycocyanin, different pigments of the carotenoid groups) and qualitative data on the presence of hydrocarbons and oil pollutants. Sea water samples from different depths have been collected and analyzed from August 2010 through November 2011 in the Gulf of Asinara (N-W Sardinia). Several sampling stations were selected as sites with different degree of pollution. The accuracy and the reliability of data obtained by CASPER have been evaluated comparing the results with other standard measurements such as: Chlorophyll a (Chl a) data obtained by spectrophotometric method and total phytoplankton abundance in terms of density and class composition. Spectral deconvolution technique was developed and integrated with CASPER system to assess and characterize a marker pigments and organic compounds in situ and in vivo. Field studies confirmed CASPER system capability to effectively discriminate characteritistic spectra of fluorescent water constituents, contributing to decrease the time-consuming manual analysis of the water samples in the laboratory.

Item Type:Article
ID Code:9623
Status:Published
Refereed:Yes
Uncontrolled Keywords:Laser excitation, deconvolution, chlorophyll-a, algal pigments, environmental monitoring, Sardinia
Subjects:Area 05 - Scienze biologiche > BIO/07 Ecologia
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > InTreGA s.r.l spin-off ENEA, Sassari
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Architettura, Design e Urbanistica
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze della Natura e del Territorio
Publisher:Scientific Research Publishing
ISSN:2161-7384
eISSN:2161-7392
Copyright Holders:© Maria Sighicelli et al., © are reserved for SCIRP and the owner of the intellectual property Maria Sighicelli et al. All Copyright © are guarded by law and by SCIRP as a guardian
Deposited On:14 Feb 2014 12:03

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