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Pichia fermentans dimorphic changes depend on the nitrogen source

Sanna, Maria Lina and Zara, Severino and Zara, Giacomo and Migheli, Quirico and Budroni, Marilena and Mannazzu, Ilaria Maria (2012) Pichia fermentansdimorphic changes depend on the nitrogen source. Fungal Biology, Vol. 116 (7), p. 769-777. ISSN 1878-6146. Article.

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DOI: 10.1016/j.funbio.2012.04.008

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Abstract

Pichia fermentans DiSAABA 726 is a biofilm-forming yeast that undergoes dimorphic transition. Under yeast-like morphology it controls brown rot caused by Monilia spp. on apple fruit, while under pseudohyphal form, it shows pathogenic behaviour itself on peach fruit. The present study investigates the nutritional factors that induce and separate yeast-like and pseudohyphal morphologies under laboratory conditions. We show that P. fermentans DiSAABA 726 produces mainly yeast-like cells on media containing millimolar concentrations of urea and diammonium phosphate, and forms pseudohyphae at micromolar concentrations of these two salts. With ammonium sulphate, yeast-like or pseudohyphal morphology depends on the N concentration and the pH of the culture media. Amino acids such as methionine, valine, and phenylalanine invariably induce pseudohyphal morphology irrespective of the N concentration and the pH of the culture media. Methionol, 1-butanol, isobutanol, and isopropanol induce pseudohyphal growth, while phenylethanol and isoamyl alcohol fail to induce the formation of filaments. Thus, the morphogenesis of P. fermentans DiSAABA 726 depends more on the nitrogen source than on the N concentration, and is regulated by the quorum-sensing molecules that are generally produced from amino-acid assimilation under nitrogen starvation.

Item Type:Article
ID Code:9572
Status:Published
Refereed:Yes
Uncontrolled Keywords:Biofilm, nitrogen starvation, Pichia fermentans, Pseudohyphae, yeast morphogenesis
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto nazionale biostrutture e biosistemi, Unità di ricerca, Sassari
Publisher:Elsevier
ISSN:1878-6146
Copyright Holders:© 2012 The British Mycological Society
Deposited On:15 Jan 2014 11:15

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