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Electrophoretic karyotypes of Fusarium spp.

Migheli, Quirico and Berio, Tilde and Gullino, Maria Lodovica (1993) Electrophoretic karyotypes of Fusarium spp. Experimental Mycology, Vol. 17 (4), p. 329-337. ISSN 0147-5975. eISSN 1878-4399. Article.

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DOI: 10.1006/emyc.1993.1031

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Abstract

The electrophoretic karyotype of 17 antagonistic and pathogenic strains of Fusarium spp. has been established by using contour-clamped homogeneous electric field gel electrophoresis. Intact chromosomal DNA was prepared from fungal protoplasts with standard procedures. Up to 11 distinct chromosomal bands were resolved after 184 h of migration at 50 V. Polymorphic karyotypes were observed in different species of Fusarium, formae speciales of F. oxysporum, and races of F. oxysporum f.sp. dianthi. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards, the size of the Fusarium genome was estimated to range from approximately 18.1 to 51.5 Mb. The suitability of electrophoretic karyotyping as a tool for strain characterization, as well as some applications in hybridization analysis of Fusarium spp., is discussed.

Item Type:Article
ID Code:9570
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fusarium spp., chromosomal DNA, electrophoretic karyotyping, contour-clamped homogenous electric field (CHEF) gel electrophoresis
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
ISSN:0147-5975
eISSN:1878-4399
Additional Information:Poi Fungal genetics and biology.
Deposited On:15 Jan 2014 10:01

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