Lamon, Sonia and Mureddu, Anna and Marceddu, Marta and Consolati, Simonetta Gianna and Mazza, Roberta and Meloni, Domenico and Mazzette, Rina (2011) Ricerca diretta di VTEC in molluschi bivalvi provenienti da un banco naturale. In: 13. Congresso Nazionale S.I.Di.L.V., 12-14 ottobre 2011, Trani, Italia. Parma, [Società Italiana di Diagnostica di Laboratorio Veterinaria - S.I.Di.L.V.]. p. 245-246. Conference or Workshop Item.
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The aim of the present study was to improve a molecular screening test for the direct detection of VTEC in shellfish, collected from a Sardinian growing natural bed and to define the virulence profile of the isolates. Thirty samples of shellfish were analyzed by PCR. Virulence factors of the strains (isolated by IMS) were determined by multiplex PCR. The prevalence of VTEC by direct PCR screening test was 6.6%. According to the multiplex PCR, the DNA from two enrichment broths showed a complete virulence profile, instead in all the strains isolated by culture methods any virulence genes were detected.
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