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Evaluation of the rapid assay Betastar Combo 3.0 for the detection of Penicillin, Amoxicillin, Cefazolin and Oxytetracycline in individual sheep milk

Pazzola, Michele and Piras, Gianpiera and Noce, Antonia and Dettori, Maria Luisa and Vacca, Giuseppe Massimo (2015) Evaluation of the rapid assay Betastar Combo 3.0 for the detection of Penicillin, Amoxicillin, Cefazolin and Oxytetracycline in individual sheep milk. Small Ruminant Research, Vol. 124 , p. 127-131. ISSN 0921-4488. eISSN 1879-0941. Article.

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DOI: 10.1016/j.smallrumres.2015.01.001

Abstract

The aim of this study was to evaluate a 3-min assay (Betastar® Combo 3.0) for the detection of Penicillin G, Amoxicillin, Cefazolin and Oxytetracycline hydrochloride in individual ewe milk. Antibiotics-free samples were obtained throughout three trials from three Sarda ewes. At the laboratory, samples were spiked with the four antimicrobial molecules at different concentrations. These were on the basis of the specific maximum residue limits fixed by the European Union (MRLs): 4 μg/kg for Penicillin G, 4 μg/kg for Amoxicillin, 50 μg/kg for Cefazolin and 100 μg/kg for Oxytetracycline. Negative control samples (180 tests) were always detected as negative. The limit of detection for Penicillin was lower than the MRL, whereas for Amoxicillin and Oxytetracycline it was slightly higher than the MRL in one case out of nine examinations. The limit of detection for Cefazolin was higher than the MRL. In conclusion, Betastar® Combo 3.0 was suitable for rapid screening of individual sheep milk spiked with Penicillin G, Amoxicillin and Oxytetracycline.

Item Type:Article
ID Code:9562
Status:Published
Refereed:Yes
Uncontrolled Keywords:Antimicrobial residue, detection test, sheep milk
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
ISSN:0921-4488
eISSN:1879-0941
Deposited On:16 Feb 2015 14:52

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