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Differentiation between fiber and drug types of hemp (Cannabis sativa L.) from a collection of wild and domesticated accessions

Piluzza, Giovanna Antonia Maria and Delogu, Giovanna and Cabras, Antonietta and Marceddu, Salvatore and Bullitta, Simonetta Maria (2013) Differentiation between fiber and drug types of hemp (Cannabis sativa L.) from a collection of wild and domesticated accessions. Genetic Resources and Crop Evolution, Vol. 60 (8), p. 2331-2342. ISSN 0925-9864. Article.

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DOI: 10.1007/s10722-013-0001-5

Abstract

Accessions of wild and domesticated hemp (Cannabis sativa L.) originating from Colombia, Mexico, California, Bolivia, Thailand, Afghanistan, Serbia, Hungary, south Africa and different regions of China, were studied by means of DNA polymorphisms in order to discriminate between drug and fiber types. Analysis of molecular variance (AMOVA) was used to partition the total genetic variance within and among populations. The significance of the variance components was tested by calculating their probabilities based on 999 random permutations. AMOVA revealed 74 % variation among accessions and 26 % within accessions, all AMOVA variation was highly significant (P < 0.001). The cluster analysis of molecular data, grouped accessions into eight clusters and gave a matrix correlation value of r = 0.943, indicating a very good fit between the similarity values implied by the phenogram and those of the original similarity matrix. In this study, DNA polymorphisms could discriminate the fiber and drug types, and accessions were grouped in accordance to their classification and uses. In addition, seed size variation and micromorphological characters of seeds were studied by means of a scanning electron microscope (SEM). Seeds varied significantly in size, and were bigger in the fiber types. SEM analysis exhibited variation of micromorphological characters of seeds that could be important for discriminating the fiber or drug types.

Item Type:Article
ID Code:9557
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cannabis sativa L., DNA polymorphisms, drug type, fiber type, seeds, SEM
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/02 Agronomia e coltivazioni erbacee
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto per il sistema produzione animale in ambiente Mediterraneo, Sassari
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di scienze delle produzioni alimentari, Sassari
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di chimica biomolecolare, Sassari
Publisher:Springer Netherlands
ISSN:0925-9864
Copyright Holders:© Springer Science+Business Media Dordrecht 2013
Deposited On:18 Dec 2013 15:47

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