Vargiu, Silvia (2014) Microgravity and immune system: phosphorylative modifications related to the recognition of apoptotic cells by phagocytes in simulated microgravity. Doctoral Thesis.
This thesis deals with experiments on phosphorylative changes in peripheral blood mononuclear cell cultures grown under simulated microgravity conditions and in normal conditions. Proteomic techniques have been used to evaluate phosphorylative post-translational modifications and to compare the protein pattern of cells grown in a microgravity environment simulated by the Random Positioning Machine. Monodimensional electrophoresis was used to visualize the proteins of interest obtained from peripheral blood mononuclear cells; Blue Coomassie staining was performed to visualize proteins. MALDI-ToF mass spectrometry was also employed to characterize the same proteins. Western Blotting with specific antibodies: antiphosphotyrosine and anti-Syk was prepared to study phosphorylative differences between cells grown in normal conditions (1xg), those grown under simulated microgravity (0xg) and activated by a gravisensitive mitogen such as Concanavalina A.
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