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Association of Trichomonas vaginalis with its symbiont Mycoplasma hominis synergistically upregulates the in vitro proinflammatory response of human monocytes

Fiori, Pier Luigi and Diaz, Nicia Maria Luisa Eugenia and Cocco, Anna Rita and Rappelli, Paola and Dessì, Daniele (2013) Association of Trichomonas vaginalis with its symbiont Mycoplasma hominis synergistically upregulates the in vitro proinflammatory response of human monocytes. Sexually Transmitted Infections, Vol. 89 (6), p. 449-454. ISSN 1368-4973. eISSN 1472-3263. Article.

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DOI: 10.1136/sextrans-2012-051006

Abstract

Objectives Trichomonas vaginalis is the causative agent of trichomoniasis, one of the most common sexually transmitted diseases worldwide. In recent years we have described the symbiotic relationship between T. vaginalis and Mycoplasma hominis. How this biological association might affect the pathogenicity of one or both the microorganisms is still unknown. Since local inflammation is thought to play a central role in T. vaginalis infection, we investigated the in vitro response of human macrophages to naturally mycoplasma-free T. vaginalis, as compared to a mycoplasma-infected trichomonad isolate.
Methods THP-1 cells were stimulated with two isogenic T. vaginalis isolates, one naturally mycoplasma-free and one stably associated with M. hominis, and secreted cytokines measured by ELISA. Nuclear factor κB (NFκB) involvement in THP-1 response to T. vaginalis and M. hominis was evaluated by means of a reporter system based on detection of alkaline phosphatase activity.
Results We found that the presence of M. hominis upregulates the expression of a panel of proinflammatory cytokines in a synergistic fashion. We also found that the upregulation of the proinflammatory response by THP-1 cells involves the transcription factor NFκB.
Conclusions These findings suggest that the presence of M. hominis in T. vaginalis isolates might play a key role in inflammation during trichomoniasis, thus affecting the severity of the disease. The synergistic upregulation of the macrophage proinflammatory response might also affect some important clinical conditions associated with T. vaginalis infection, such as the increased risk of acquiring cervical cancer or HIV, which are thought to be affected by the inflammatory milieu during trichomoniasis.

Item Type:Article
ID Code:9347
Status:Published
Refereed:Yes
Uncontrolled Keywords:Micoplasma, Trichomonas, inflammation
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:BMJ
ISSN:1368-4973
eISSN:1472-3263
Deposited On:26 Nov 2013 09:36

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