Addis, Maria Filippa and Rapelli, Paola and Pinto de Andrade, Ana Maria and Madeira Rita, Fatima and Colombo, Mauro Maria and Cappuccinelli, Pietro Antonio and Fiori, Pier Luigi (1999) Identification of Trichomonas vaginalis alpha-actinin as the most common immunogen recognized by sera of women exposed to the parasite. The Journal of Infectious Diseases, Vol. 180 (5), p. 1727-1730. ISSN 0022-1899. Article.
A study on presence of antibodies to Trichomonis vaginalis in serum was done on a group of 500 pregnant, asymptomatic Angolan women. A serologic screening, done by ELISA, revealed that 41% of the women had IgG and IgM against the parasite. Analysis of sera by immunoblotting revealed that 94.4% of sera with anti-T. vaginalis IgG class antibodies were reactive against a common immunogenic protein of 115 kDa. The common immunogen was identified as the protozoan α-actinin. All sera recognizing the 115-kDa antigen were reactive against both native and recombinant T. vaginalis α-actinin and nonreactive against human α-actinin. The findings presented in this work offer a new tool for epidemiologic studies and open new perspectives for vaccination.
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