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The Effect of sperm preparation and brief gamete coincubation length on ovine oocytes fertilization, subsequent development and embryo quality

Ali, A. and Pasciu, Valeria and Bomboi, Giovanni Cristoforo and Floris, Basilio Remo and Naitana, Salvatore (2012) The Effect of sperm preparation and brief gamete coincubation length on ovine oocytes fertilization, subsequent development and embryo quality. Reproduction in Domestic Animals, Vol. 47 (Supplement s4), p. 517. eISSN 1439-0531. Article.

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DOI: 10.1111/j.1439-0531.2012.02119.x

Abstract

Over the past 70 years, egg yolk (EY) is frequently used as a cryoprotectant in semen diluents and has been confirmed highly effective for the maintenance of sperm fertility. However, the preparation of uniform diluents containing EY is hard. Since Cheng, (1985) reported the beneficial effect of overnight gamete oocytes coincubation length in IVF, this protocol was used a recommended protocol in several species. However, this protocol leading to exposure of oocytes and embryos to toxic effects associated with excessive generation of reactive oxygen species. The aims of this study were to examine (i) whether brief exposure of mature ewe oocytes to frozen-thawed semen prepared with different semi-defined diluents may affect fertilization, enhanced embryonic development, embryo quality before and/or after freezing vs. long coincubation length, and (ii) explore the accompanying effects of different semi-defined diluents and coincubation length on fertilization and embryo development rates.

Item Type:Article
ID Code:9157
Status:Published
Refereed:Yes
Uncontrolled Keywords:Semi-defined semen diluents, in vitro fertilization, brief coincubation length, embryonic development and quality
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Blackwell / Wiley
eISSN:1439-0531
Copyright Holders:© 2012 Blackwell Verlag GmbH
Additional Information:Abstract presented at the 17. International Congress on Animal Reproduction (ICAR), Vancouver, Canada, 29 July-2 August 2012.
Deposited On:23 Jul 2013 08:54

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