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Zinc transporter 8 and MAP3865c homologous epitopes are recognized at T1D onset in Sardinian children

Masala, Speranza and Zedda, Maria Antonietta and Cossu, Davide and Ripoli, Carlo and Palermo, Mario (2013) Zinc transporter 8 and MAP3865c homologous epitopes are recognized at T1D onset in Sardinian children. PLoS One, Vol. 8 (5), e63371. eISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0063371

Abstract

Our group has recently demonstrated that Mycobacterium avium subspecies paratuberculosis (MAP) infection significantly associates with T1D in Sardinian adult patients. Due to the potential role played by MAP in T1D pathogenesis, it is relevant to better characterize the prevalence of anti-MAP antibodies (Abs) in the Sardinian population, studying newly diagnosed T1D children. Therefore, we investigated the seroreactivity against epitopes derived from the ZnT8 autoantigen involved in children at T1D onset and their homologous sequences of the MAP3865c protein. Moreover, sera from all individuals were tested for the presence of Abs against: the corresponding ZnT8 C-terminal region, the MAP specific protein MptD, the T1D autoantigen GAD65 and the T1D unrelated Acetylcholine Receptor. The novel MAP3865c281–287 epitope emerges here as the major C-terminal epitope recognized. Intriguingly ZnT8186–194 immunodominant peptide was cross-reactive with the homologous sequences MAP3865c133–141, strengthening the hypothesis that MAP could be an environmental trigger of T1D through a molecular mimicry mechanism. All eight epitopes were recognized by circulating Abs in T1D children in comparison to healthy controls, suggesting that these Abs could be biomarkers of T1D. It would be relevant to investigate larger cohorts of children, followed over time, to elucidate whether Ab titers against these MAP/Znt8 epitopes wane after diagnosis.

Item Type:Article
ID Code:9067
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium avium subspecies paratuberculosis (MAP) infection, T1D pathogenesis, ZnT8 autoantigen, MAP3865c protein, Sardinia
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Public Library of Science
eISSN:1932-6203
Copyright Holders:© 2013 Masala et al.
Deposited On:29 May 2013 11:58

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