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Quantification of arginine and dimethylated arginines in human plasma by field-amplified sample injection capillary electrophoresis UV detection

Zinellu, Angelo and Sotgia, Salvatore and Deiana, Luca and Carru, Ciriaco (2013) Quantification of arginine and dimethylated arginines in human plasma by field-amplified sample injection capillary electrophoresis UV detection. In: Volpi, Nicola and Maccari, Francesca (eds). Capillary electrophoresis of biomolecules: methods and protocols. [S.l.], Humana Press. p. 131-138. (Methods in molecular biology, 984). ISBN 978-1-62703-295-7. eISBN 978-1-62703-296-4. Book Section.

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DOI: 10.1007/978-1-62703-296-4_10

Abstract

We report a capillary electrophoresis method by field-amplified sample injection (FASI) for the separation and detection of free plasma arginine and dimethylated arginines. Protein elimination from plasma was performed by acetonitrile/ammonia precipitation. Supernatants were dried, reswollen in water, and directly injected on capillary without complex cleanup by solid-phase extraction and/or tedious sample derivatization procedures. Analytes were baseline-separated within 22 min by using 50 mmol/L Tris phosphate pH 2.3 as running buffer. Due to the stacking effects of electrokinetic injection, it is possible to perform a considerable on-line preconcentration of the analytes before running the electrophoresis. This procedure allows to reach a detection limit in real sample of 10 nmol/L for dimethylated arginines and 20 nmol/L for arginine. Recovery of plasma ADMA was 99–104 % and inter-day CV was less than 3 %.

Item Type:Book Section
ID Code:9058
Status:Published
Refereed:Yes
Uncontrolled Keywords:Capillary electrophoresis, FASI, ADMA, SDMA arginine
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Humana Press
Copyright Holders:© Springer Science + Business Media, LLC 2013
ISBN:978-1-62703-295-7
ISBN:978-1-62703-296-4
Deposited On:16 May 2013 13:25

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