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Gene dosage effect of WEE1 on growth and morphogenesis from arabidopsis hypocotyl explants

Spadafora, Natasha D. and Perrotta, Lara and Nieuwland, Jeroen and Albani, Diego Maria and Bitonti, Maria Beatrice and Herbert, Robert J. and Doonan, John H. and Marchbank, Angela M. and Siciliano, Ilario and Grønlund, Anne Lentz and Francis, Dennis and Rogers, Hilary J. (2012) Gene dosage effect of WEE1 on growth and morphogenesis from arabidopsis hypocotyl explants. Annals of Botany, Vol. 110 (8), p. 1631-1639. eISSN 1095-8290. Article.

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DOI: 10.1093/aob/mcs223

Abstract

Background and Aims How plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage effect on growth and development in Arabidospis thaliana. To do this, a comparison was made of the development of gain- and loss-of-function WEE1 arabidopsis lines both in vivo and in vitro.
Methods Hypocotyl explants from an over-expressing Arath;WEE1 line (WEE1oe), two T-DNA insertion lines (wee1-1 and wee1-4) and wild type (WT) were cultured on two-way combinations of kinetin and naphthyl acetic acid. Root growth and meristematic cell size were also examined.
Key Results Quantitative data indicated a repressive effect in WEE1oe and a significant increase in morphogenetic capacity in the two T-DNA insertion lines compared with WT. Compared with WT, WEE1oe seedlings exhibited a slower cell-doubling time in the root apical meristem and a shortened primary root, with fewer laterals, whereas there were no consistent differences in the insertion lines compared with WT. However, significantly fewer adventitious roots were recorded for WEE1oe and significantly more for the insertion mutant wee1-1. Compared with WT there was a significant increase in meristem cell size in WEE1oe for all three ground tissues but for wee1-1 only cortical cell size was reduced.
Conclusions There is a gene dosage effect of WEE1 on morphogenesis from hypocotyls both in vitro and in vivo.

Item Type:Article
ID Code:9054
Status:Published
Refereed:Yes
Uncontrolled Keywords:Arabidopsis thaliana, cell cycle, development, growth, hypocotyl, tissue culture, WEE1
Subjects:Area 05 - Scienze biologiche > BIO/04 Fisiologia vegetale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze della Natura e del Territorio
Publisher:Oxford University Press
eISSN:1095-8290
Copyright Holders:© The Author 2012
Deposited On:16 May 2013 11:13

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