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An NMR study on nickel binding to Cap43 protein

Zoroddu, Maria Antonietta and Peana, Massimiliano Francesco and Medici, Serenella (2006) An NMR study on nickel binding to Cap43 protein. In: SCI 2006: 22. Congresso nazionale della Società Chimica Italiana: atti del congresso, 10-15 settembre 2006, Firenze, Italia. [S.l.], Società Chimica Italiana. p. 405. Conference or Workshop Item.

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Cap43 has been reported to be specifically induced by nickel compounds in a variety of cell lines. Its function is not yet clear, but Cap43 protein does appear to be inducec in response to an increase in intracellular concentration of Ca2+, caused by nickel ion exposure in cultured human cells. Cap43 is expressed at low levels in normal tissues. However, in a variety of cancers, it is overexpressed in cancer cells.
The peculiarity of Cap43 is its mono-histidinic motif consisting of ten amino acids (TRSRHTSEG) repeated three times in the C-terminus.
We have analyzed, for Ni(II) binding, the 30-amino acid C-terminal sequence of the protein, TRSRSHTSEG-TRSRTHTSEG-TRSRSHTSEG, by the use of different NMR techniques such as 1D, NOESY, TOCSY and ROESY experiments. From the data thus collected we calculated a model of the structure for the peptide-Ni(II) complex, confirming the characteristic binding of a nickel ion to each 10-amino acid fragment and showing the interesting structural changes the peptide undergoes upon metal coordination.

Item Type:Conference or Workshop Item (Poster)
ID Code:8974
Uncontrolled Keywords:Nuclear Magnetic Resonance spectroscopy, metal complex, cap43
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:Società Chimica Italiana
Publisher Policy:Depositato per gentile concessione dell'Editore
Deposited On:06 May 2013 10:58

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