Khadjavi, Amina and Notarpietro, Agata and Mannu, Franca Rita and Pantaleo, Antonella and Ferru, Emanuela and Destefanis, Paolo and Fontana, Dario and Turrini, Francesco Michelangelo (2013) A High-throughput assay for the detection of Tyr-phosphorylated proteins in urine of bladder cancer patients. Biochimica et Biophysica Acta. General Subjects, Vol. 1830 (6), p. 3664-3669. ISSN 0304-4165. Article.
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Background Bladder cancer has the peculiarity of shedding neoplastic cells and their components in urine representing a valuable opportunity to detect diagnostic markers. Using a semi-quantitative method we previously demonstrated that the levels of Tyr-phosphorylated proteins (TPPs) are highly increased in bladder cancer tissues and that soluble TPPs can also be detected in patient's urine samples. Although the preliminary evaluation showed very promising specificity and sensitivity, insufficient accuracy and very low throughput of the method halted the diagnostic evaluation of the new marker. To overcome this problem we developed a quantitative methodology with high sensitivity and accuracy to measure TPPs in urine.
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