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Interaction of divalent cations with Park9 protein fragments

Remelli, Maurizio and Peana, Massimiliano Francesco and Medici, Serenella and Anedda, Roberto and Zoroddu, Maria Antonietta (2012) Interaction of divalent cations with Park9 protein fragments. In: ISMEC 2012: 23. International symposium on metal complexes, 18-22 June 2012, Lisbon, Portugal. [S.l.], ISMEC Group / Universidade Técnica de Lisboa. p. 191-192. (ISMEC GROUP SERIES, 2). ISSN 2239-2459. Conference or Workshop Item.

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Abstract

Two peptide sequences from Park9 Parkinson’s disease (PD) gene, -P1D2E3K4H5E6L7- (1) and -F1C2G3D4G5A6N7D8C9G10- (2) have been tested for Mn(II), Zn(II) and Cu(II) binding. Park9 encoded protein can protect cells from manganese poisoning, which is an environmental risk factor for a Parkinson’s disease-like syndrome [1-4]. In fact, Park9 belongs to a family of ATP-ases involved in metal coordination and transportation; familial gene mutations may result in early development of PD. The chosen fragments are located from 1165 to 1171 and from 1184 to 1193 residues in the Park9 sequence, and are highly conserved in a number of organisms, going from yeasts to humans. Potentiometric, UV-vis experiments together with mono- and multidimensional NMR spectroscopy have been used to understand the details of metal binding sites at different pH values and at different ligand to metal molar ratios, showing that the three metals are able to effectively bind the examined peptides.
From NMR measurements Mn(II) and Zn(II) coordination with peptide 1 involves imidazole Nε or Nδ of His5 and carboxyl γ-O of Asp2, Glu3 and Glu6 residues. Six donor atoms participate in Mn(II) binding, resulting in a distorted octahedral geometry, possibly involving bidentate interaction of carboxyl groups; four donor atoms participate in Zn(II) binding, resulting in a tetracoordinated geometry. Potentiometric data show that soluble, hydroxylated Zn(II) species are formed in the alkaline pH range. The formation of Cu(II) complexes with peptide 1 starts below pH: only mononuclear complexes have been potentiometrically detected also in the presence of excess of ligand. Imidazole nitrogen of His residues acts as first Cu(II) anchoring site; as pH is raised, ligand coordination proceeds with deprotonation and binding of neighbouring amide nitrogens of the peptidic backbone. UV-vis spectra agree that the main species at neutral pH is a {Nim, 2N-, O} complex, where the oxygen atom most likely belongs to an equatorially coordinated water molecule.
Cu(II), Mn(II) and Zn(II) coordination involves the cysteine residues with peptide 2 and complex-formation invariably starts at lower pH with respect to ligand 1. Mn(II) accepts additional ligand bonds from D4 and D8 to complete the coordination sphere; the unoccupied sites may contain solvent molecules. When the ligand is in excess, both Zn(II) and Cu(II) ions form bis-complexes. The two metal ions behave in a very similar way and the stoichiometry of main species at physiological pH depends on the metal/ligand ratio: [ML]2- in equimolar solution or [MHL2]5- for the 1:2 ratio. Potentiometric data suggest for the former a {2S, 2O} and for the latter a {3S, 1O} coordination without any participation of amide nitrogens, as usually found for Cu(II)/peptide complexes.

Item Type:Conference or Workshop Item (Poster)
ID Code:8922
Status:Published
Uncontrolled Keywords:Park9, complexes, manganese(II), Parkinson
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
Publisher:ISMEC Group / Universidade Técnica de Lisboa
ISSN:2239-2459
Publisher Policy:Authors retain the copyrights of their acta, and their unrestricted use, distribution and reproduction in any medium are permitted, provided that the original work is properly cited
Deposited On:03 May 2013 09:58

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