Menghini, Luigi and Leporini, Lidia and Scanu, N. and Pintore, Giorgio Antonio Mario and Ferrante, Claudio and Recinella, Lucia and Orlando, Giustino and Vacca, Michele and Brunetti, Luigi (2012) Potenzialità immunomodulanti in vitro di una formulazione a base di estratti vegetali. Minerva medica, Vol. 103 (1), p. 13-21. ISSN 0026-4806. eISSN 1827-1669. Article.
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Aim. Aim of this study was to evaluate the effects of phytocomplexes of Uncaria, Shitake and Ribes in terms of viability and inflammatory response on immune cell-derived cultures.
Methods. Standardized extracts of Uncaria, Shitake and Ribes and their commercial formulation were tested on cell lines PBMC, U937 and macrophage. The activity was evaluated in terms of cell viability (MTT test), variations of oxidative markers release (ROS and PGE2) and modulatory effects on immune response (RT-PCR expression of IL-6, IL-8 and TNFα).
Results. Cell viability was not affected by extracts, except subtle variations observed only at higher doses (>250 μg/mL). The extract mixture was well tolerated, with no effects on cell viability up to doses of 500 μg/mL. Pre-treatment of macrophages with subtoxic doses of the extracts reduced the basal release of oxidative markers and enhanced the cell response to oxogenus oxidant stimulation, as revealed by ROS and PGE2 release reduction. The same treatment on macrophage resulted in a selective modulation of the immune response, as shown by an increase of IL-6 mRNA and, partially, IL-8 mRNA, while a reduction was observed for TNFα mRNA.
Conclusion. Data confirm that extracts and their formulations can act as regulator of the immune system with mechanisms involving the oxidative stress and the release of selected proinflammatory cytokines.
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