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Interaction of divalent cations with protein PARK9

Zoroddu, Maria Antonietta and Peana, Massimiliano Francesco and Medici, Serenella and Solinas, Costantino and Juliano, Claudia Clelia Assunta and Remelli, Maurizio (2013) Interaction of divalent cations with protein PARK9. In: 12. International symposium on metal ions in biology and medicine: abstracts and proceedings, March 11-13, 2013, Punta del Este, Uruguay. Montevideo, [s.n.]. p. 39. ISBN 978-9974-0-0911-0. Conference or Workshop Item.

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Abstract

Metals have been shown to play a role in the genesis and development of many neurodegenerative diseases. Park9 encoded protein can protect cells from manganese poisoning, an environmental risk factor for a Parkinson’s disease- like syndrome. Park9 belongs to a family of ATP-ases involved in metal coordination and transportation; familial mutations of this gene may result in early development of PD. We tested two peptide sequences from Park9, -P1D2E3K4H5E6L7- (1) and -F1C2G3D4G5A6N7D8C9G10- (2), for Mn(II), Zn(II) and Cu(II) binding. These fragments are located from 1165 to 1171 and from 1184 to 1193 residues in Park9 sequence, and are highly conserved in a number of organisms, from yeasts to humans. Experiments have been carried out at different pH values and ligand/metal molar ratios with both potentiometric and spectroscopic (NMR, UV-vis) techniques, showing that the three metals are able to effectively bind the examined peptides. Mn(II) and Zn(II) coordination with peptide (1) involves imidazol of His5 and carboxyl γ-O of Asp2, Glu3 and Glu6 residues, in a distorted octahedral geometry, possibly involving bidentate interaction of carboxyl groups; four donor atoms participate in Zn(II) binding, resulting in a tetracoordinated geometry. Mn(II) and Zn(II) coordination involves the two cysteines in peptide (2); Mn(II) accepts additional ligand bonds from D4 and D8 to complete the coordination sphere, together with some water molecules. Details of Cu(II) coordination are under study.

Item Type:Conference or Workshop Item (Lecture)
ID Code:8873
Status:Published
Uncontrolled Keywords:Parkinson's disease, Park9 protein, divalent cations
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
Publisher:[s.n.]
ISBN:978-9974-0-0911-0
Deposited On:22 Apr 2013 08:55

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