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In vivo characterisation of a Nafion®-modified Pt electrode for real-time nitric oxide monitoring in brain extracellular fluid

Finnerty, Niall J. and O'Riordan, Saidhbhe L. and Brown, Finbar O. and Serra, Pier Andrea and O'Neill, Robert D. and Lowry, John P. (2012) In vivocharacterisation of a Nafion®-modified Pt electrode for real-time nitric oxide monitoring in brain extracellular fluid. Analytical Methods, Vol. 4 (2), p. 550-557. eISSN 1759-9679. Article.

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DOI: 10.1039/C2AY05924J

Abstract

A Nafion®-modified Pt sensor developed for real-time neurochemical monitoring in freely-moving animals has now been characterised in vivo for the detection of nitric oxide (NO). Experiments were undertaken to test sensitivity, selectivity and stability. In control experiments, local administration of aCSF resulted in a decrease in signal (42 ± 12 pA, n = 9), while systemic administration of saline caused a transient increase in signal (22 ± 3 pA, n = 9) from baseline levels. Local MD perfusions of known concentrations of NO (1 mM) and L-arginine (720 μM) caused significant increases in current (4 ± 1 nA (n = 7) and 271 ± 37 pA (n = 5) respectively) while systemic administration of the non-selective NOS inhibitor L-NAME produced a significant decrease in current (91 ± 19 pA, n = 4) compared to baseline levels. Confirmation that the sensor remained selective was achieved by injection of ascorbic acid (2 g kg−1, i.p). The oxidation current showed no variation over the selected time interval of 60 min, indicating no deterioration of the polymer membrane. Finally, after an initial decrease in sensitivity (ca. 50%) within the first 24 h of implantation the Nafion®-modified Pt sensor showed excellent stability under physiological conditions over extended periods (8 days) validating its use for continuous chronic in vivo recordings.

Item Type:Article
ID Code:8848
Status:Published
Refereed:Yes
Uncontrolled Keywords:Nafion®-modified Pt electrode, nitric oxide (NO), brain extracellular fluid
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:Royal Society of Chemistry
eISSN:1759-9679
Deposited On:20 May 2013 16:55

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