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Genotypic and phenotypic features of enteropathogenic Escherichia coli isolated in industrialized and developing countries

Santona, Simona and Diaz, Nicia Maria Luisa Eugenia and Fiori, Pier Luigi and Francisco, Moises and Sidat, Mohsin and Cappuccinelli, Pietro Antonio and Rappelli, Paola (2013) Genotypic and phenotypic features of enteropathogenic Escherichia coli isolated in industrialized and developing countries. The Journal of Infection in Developing Countries, Vol. 7 (3), p. 214-219. eISSN 1972-2680. Article.

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DOI: 10.3855/jidc.3054

Abstract

Introduction: Typical EPEC are considered a leading cause of diarrhoea in developing countries, while atypical EPEC have been isolated more frequently in developed areas. The actual geographic distribution of the two EPEC subgroups is controversial, since data can be highly influenced by laboratory resources. This study aimed to compare the distribution of typical and atypical EPEC among children in developed and developing countries, and to characterize the bacterial isolates, using a unique methodological approach.
Methodology: A total of 1,049 E. coli were isolated from faeces of children with acute diarrhoea in Mozambique, Angola and Italy, and processed by PCR to assess the presence of a large panel of virulence genes. All isolates classified as EPEC were further characterized by evaluating adherence and capability to induce actin rearrangement on Hep-2 cells.
Results: Overall we isolated 59 EPEC, likewise distributed in the three countries, representing the 5.04%, 4.44% and 6.97% of all Mozambican, Angolan and Italian isolates, respectively. Nevertheless, the geographic distribution of the two EPEC subgroups was not homogeneous: in Italy we isolated 28 aEPEC but no tEPEC, while in Angola and Mozambique the percentage of the two subgroups was comparable. Twelve atypical EPEC were FAS positive and able to induce localized-like adherence on Hep-2 cells, but no correlation with the geographic origin of isolates was observed.
Conclusion: Atypical EPEC are present in sub-Saharan areas in a percentage similar to that of typical strains, and are not mainly restricted to industrialized countries, as it was previously supposed.

Item Type:Article
ID Code:8802
Status:Published
Refereed:Yes
Uncontrolled Keywords:Diarrhoeagenic E. coli, atypical EPEC, Italy, Mozambique, Angola
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:OLoeP
eISSN:1972-2680
Copyright Holders:© 2013 Santona et al.
Deposited On:20 Mar 2013 16:46

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