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Susceptibility of various corneal fungal isolates and Pseudomonas aeruginosa to contact lens disinfecting solutions

Pinna, Antonio <1963- > and Usai, Donatella and Zanetti, Stefania Anna Lucia and Thomas, Philip A. and Kaliamurthy, Jayaraman (2012) Susceptibility of various corneal fungal isolates and Pseudomonas aeruginosa to contact lens disinfecting solutions. The Journal of Infection in Developing Countries, Vol. 7 (3), p. 261-268. eISSN 1972-2680. Article.

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DOI: 10.3855/jidc.2756

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Abstract

Introduction: We aimed to investigate the susceptibility of a combined inoculum of Pseudomonas aeruginosa and different fungal strains to 6 soft contact lens disinfectants.
Methodology: One corneal isolate of P. aeruginosa and 13 corneal fungal isolates (9 Aspergillus spp, 3 Fusarium spp, 1 Curvularia sp.) were used. The following solutions were tested: Arion Cronos, Complete RevitaLens, Dua Elite, Opti-Free Express, Regard, Oxysept Comfort, and Oxysept Comfort without catalase. The effect of the solutions was assessed on a combined inoculum of P. aeruginosa plus 1 fungal strain. Suspensions of P. aeruginosa and fungi were made in the solutions (1x106 colony-forming units/mL). After 1 hour (Arion Cronos only), 6, 8, and 24 hours, aliquots of suspension were removed and seeded on Luria-Bertani and Sabouraud agar plates.
Results: After 6 hours’ exposure, all the solutions but Dua Elite and Oxysept Comfort eradicated P. aeruginosa. Conversely, apart from 3% hydrogen peroxide-based Oxysept Comfort without catalase, which eradicated all the fungi tested after 6 hours, all the other solutions were partly ineffective at killing some of the fungal isolates, even after 24 hours’ exposure.
Conclusions: Most contact lens disinfectants may be ineffective if contact lens care systems become co-contaminated with P. aeruginosa and fungi. In our experiment, only exposure to 3% hydrogen peroxide without neutralizer for at least 6 hours was always able to kill a combined inoculum of P. aeruginosa and different fungal strains.

Item Type:Article
ID Code:8800
Status:Published
Refereed:Yes
Uncontrolled Keywords:Contact lens-related keratitis, contact lens disinfectants, filamentous fungi, Pseudomonas aeruginosa co-culture, susceptibility
Subjects:Area 06 - Scienze mediche > MED/30 Malattie apparato visivo
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Chirurgiche, Microchirurgiche e Mediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:OLoeP
eISSN:1972-2680
Copyright Holders:© 2013 Pinna et al.
Deposited On:20 Mar 2013 15:54

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