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Studio proteico della parete cellulare di saccharomyces cerevisiae in condizioni di stress

Fancellu, Francesca (2009) Studio proteico della parete cellulare di saccharomyces cerevisiae in condizioni di stress. Doctoral Thesis.

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Abstract

Wine yeasts during alcoholic fermentation and ageing are subjected to several stresses and cell wall is the first compartment involved in the stress response. This PhD thesis deals with the study of the variation in cell wall protein composition in 6 different strains of Saccharomyces cerevisiae (flor/no flor wine strains and laboratory strains). We developed a mass spectrometric method (ESI-Q-TOF) to identify CWPs, unbiased with respect to the covalent linkages to the cell wall carbohydrates. We analysed cell wall protein (CWPs) composition in the studied strains in three different conditions: logarithmic phase and stationary phase in a rich fermenting medium and a flor phase in a minimal medium added with ethanol. In all conditions tested we identified overlapping sets of 20 different proteins. Among them 14 are predicted GPI-modified proteins and 6 are ASL proteins. We choose 5 interesting GPI-proteins and we used qRT-PCR to study related genes in order to identify variation in gene expressions related to different phases.

Item Type:Doctoral Thesis
ID Code:875
Publisher:Universita' degli studi di Sassari
Uncontrolled Keywords:Saccharomyces cerevisiae, parete cellulare, risposta allo stress, biofilm, vini flor
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Cicli, scuole e corsi:Ciclo 21 > Corsi > Biotecnologie microbiche agroalimentari
Deposited On:18 Aug 2009 10:03

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