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Characterization of an established endothelial cell line from primary cultures of fetal sheep hypothalamus

Mura, Emilio and Suman, Michele and Montelli, S. and Peruffo, A. and Cozzi, Bruno and Farina, Vittorio (2013) Characterization of an established endothelial cell line from primary cultures of fetal sheep hypothalamus. Research in veterinary science, Vol. 94 (3), p. 388-393. eISSN 1532-2661. Article.

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DOI: 10.1016/j.rvsc.2012.10.022

Abstract

Immortalized cell lines from fetal brain are an experimental model for studying the in vitro molecular pathways regulating neural cell differentiation and the development of neural networks. The procedures are described to obtain an established cell line from the 90-day old fetal sheep hypothalamus. Viral oncogene LT-SV40 transformation was used to isolate a stable cell line (ENOS-01) that was characterized immunocytochemically. Immortalized cells can be classified as an endothelial cell line of hypothalamic microvasculature. Furthermore, mRNA expression and immunocytochemical of estrogen receptors α and β were also evaluated. Since it is known that cerebral vessels are directly targeted by sex steroids, our established cell line represents an alternative system to study estradiol/receptor interactions during brain development. Our in vitro model can provide a tool to investigate the complex relationships among the cell types forming the blood–brain barrier, which is known to be involved in the pathogenesis of sheep transmissible neurological diseases.

Item Type:Article
ID Code:8725
Status:Published
Refereed:Yes
Uncontrolled Keywords:Established endothelial cells, transfection, hypothalamus, fetal sheep
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/01 Anatomia degli animali domestici
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
eISSN:1532-2661
Copyright Holders:© 2012 Elsevier
Deposited On:01 Mar 2013 13:09

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