Ariu, Federica and Fois, Stefano and Bebbere, Daniela and Ledda, Sergio and Rosati, Irma and Zedda, Maria Teresa and Pau, Salvatore and Bogliolo, Luisa (2012) The Effect of okadaic acid on meiotic maturation of canine oocytes of different size. Theriogenology, Vol. 77 (1), p. 46-52. eISSN 1879-3231. Article.
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The present study was conducted to determine the effect of okadic acid (OA), a potent inhibitor of seronine/treonine 1 and 2A phosphatase, on meiotic resumption and progression in canine oocytes with different diameters. Cumulus-oocyte complexes were collected from ovaries of bitches at different oestrous phases. In Experiment 1, to determine the optimal concentration of OA (0.5 or 2 μM), the oocytes were pre-incubated for 1, 3, and 20 h in TCM 199 supplemented with 20% SCE and thereafter cultured in the same medium without OA. In Experiment 2, the selected oocytes were divided into three groups according to their diameter: <110 μm, 110–120 μm, >120 μm, and pre-incubated in OA 0.5 μM for 1 h. Oocytes were cultured in vitro as previously described. After 72 h of IVM, in Experiment 1, significantly more oocytes reached MII stage with 0.5 μM for 1 h (30.8% P <0.001%) for oocytes cultured in other OA condition and in control group. In Experiment 2, OA induced a significantly higher incidence of MII oocytes in the 110–120 μm and >120 μm groups (P <0.001) compared to control group, but a significantly higher proportion of the oocytes >120 μm pre-incubated with OA progressed to MII (51.3% P <0.001). In contrast, smaller oocytes (<110) did not develop to MII stage with or without OA. In conclusion, treatment of canine oocytes with 0.5 μM for 1 h, improves meiotic maturation. The culture of fully grown (>120 μm) oocytes with OA at the onset of in vitro maturation can result in a higher frequency of meiotic maturation.
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