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Glutamyl cycle in the rat liver appears to be sex-gender specific

Campesi, Ilaria and Galistu, Adriana and Carru, Ciriaco and Franconi, Flavia and Fois, Marco and Zinellu, Angelo (2013) Glutamyl cycle in the rat liver appears to be sex-gender specific. Experimental and toxicologic pathology, Vol. 65 (5), p. 585-589. eISSN 1618-1433. Article.

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DOI: 10.1016/j.etp.2012.05.004

Abstract

Numerous studies show sexually dimorphic responses of drug metabolizing enzymes in the liver, but it is less clear whether xenobiotic detoxification mediated by glutathione is sex-gender specific. Therefore, we investigated whether sex-gender differences exist in the biosynthesis and metabolism of GSH in the rat liver.
Livers were obtained from Sprague–Dawley rats of both sexes for measurement of glutathione, its precursors and metabolites by capillary electrophoresis, whereas H2S and malondialdehyde were measured by colorimetric assays. The expression of glutamylcysteine ligase (GCL), the key enzyme in glutathione synthesis was detected by Western blotting and immunohistochemistry.
It was observed that L-methionine, glutathione, taurine and malondialdehyde (a marker of lipid peroxidation) were similar in livers from both sexes, while L-cysteine levels were significantly higher and H2S was lower in female rat livers. Furthermore, L-methionine and L-cysteine, L-cysteine and glutathione, L-cysteine and taurine were positively associated only in male livers. Finally, the female liver expressed less GCL than the male liver.
These data suggest that the glutamyl cycle in the liver is sexually dimorphic. This difference could be linked to the increased sensitivity of females to drugs and xenobiotics.

Item Type:Article
ID Code:8650
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sex-gender differences, L-cysteine, H2S, glutamylcysteine ligase, rat liver
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Urban und Fischer / Elsevier
eISSN:1618-1433
Copyright Holders:© 2012 Published by Elsevier
Deposited On:20 Feb 2013 15:34

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