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Cytochrome P450 17α-hydroxylase/C(17,20)-lyase immunoreactivity and molecular expression in the cerebellar nuclei of adult male rats

Manca, Paolo and Caria, Marcello Alessandro and Blasi, Juan and Martín Statué, Mireia and Becciu Mameli, Chiara Ombretta (2012) Cytochrome P450 17α-hydroxylase/C(17,20)-lyase immunoreactivity and molecular expression in the cerebellar nuclei of adult male rats. Journal of Chemical Neuroanatomy, Vol. 45 (1-2), p. 18-25. eISSN 1873-6300. Article.

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DOI: 10.1016/j.jchemneu.2012.07.002

Abstract

Several probes have been developed to identify steroidogenic activity in the brain of vertebrates. However, the presence of the cytochrome P450 17α-hydroxylase/C(17,20)-lyase (P450C17), an enzyme that converts pregnenolone and progesterone into dehydroepiandrosterone and androstenedione, in specific areas of the cerebellum such as the deep cerebellar nuclei, remains virtually unexplored. Using Western blot analysis and immunohistochemistry, we found molecular expression of P450C17 in the lateral, interposed and medial deep cerebellar nuclei. Moreover, double immunofluorescence procedures enabled localization of P450C17 mainly in neurons, axons and glutamatergic synapses. Taken together, these data demonstrate the occurrence of P450C17 in the deep cerebellar nuclei, and enable the chemical characterization of the cells that express the cytochrome.

Item Type:Article
ID Code:8577
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cytochrome P450C17, dehydroepiandrosterone, deep cerebellar nuclei
Subjects:Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:Elsevier
eISSN:1873-6300
Deposited On:31 Jan 2013 10:27

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