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Supragingival plaque microbial analysis in reflection to caries experience

Mannaa, Alaa and Carlén, Anette and Campus, Guglielmo Giuseppe and Lingström, Peter (2013) Supragingival plaque microbial analysis in reflection to caries experience. BMC Oral Health, Vol. 13 (5), p. 1-5. eISSN 1472-6831. Article.

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DOI: 10.1186/1472-6831-13-5

Abstract

Background: Dental caries develops as a result of the metabolism of carbohydrates by cariogenic bacteria present in a complex biofilm. The present study aimed to examine if bacteria in pooled supragingival plaque samples quantified using a “checkerboard DNA-DNA hybridization” based panel of caries-related bacteria, could reflect the caries experience in a manner similar to saliva samples analysed using a chair-side method in a previous investigation.
Methods: A total of 86 mothers and their children aged 4–6 years and 12–16 years old participated. Caries experience (DMFT/dmft; Decayed, Missing and Filled Teeth for permanent and primary teeth) was registered clinically and radiographically. Caries was recorded at the D3 level (caries into dentine). The D/d component was divided into three categories. A pooled supragingival plaque sample per participant was obtained from posterior approximal sites. Analyses of 15 bacterial species were performed using the checkerboard DNA-DNA hybridisation technique.
Results: No significant relationships were found between the bacterial scores and DMFT/dmft nor D/d groups.
Conclusions: Unlike the saliva samples and the chair-side method, interproximal pooled plaque samples analysed using the “checkerboard DNA-DNA hybridization technique” did not reveal any significant relations between the bacterial counts and the caries experience.

Item Type:Article
ID Code:8544
Status:Published
Refereed:Yes
Uncontrolled Keywords:Dental caries, saliva, supragingival plaque
Subjects:Area 06 - Scienze mediche > MED/28 Malattie odontostomatologiche
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Chirurgiche, Microchirurgiche e Mediche
001 Università di Sassari > 03 Istituti > Clinica odontoiatrica
Publisher:BioMed Central
eISSN:1472-6831
Copyright Holders:© 2013 Mannaa et al.; licensee BioMed Central Ltd.
Deposited On:24 Jan 2013 08:59

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