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Evidence for a proinflammatory and proteolytic environment in plaques from endarterectomy segments of human carotid arteries

Formato, Marilena and Farina, Miriam and Spirito, Rita and Maggioni, Marco and Guarino, Anna and Cherchi, Gian Mario and Biglioli, Paolo and Edelstein, Celina and Scanu, Angelo M. (2004) Evidence for a proinflammatory and proteolytic environment in plaques from endarterectomy segments of human carotid arteries. Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 24 (1), p. 129-135. eISSN 1524-4636. Article.

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DOI: 10.1161/01.ATV.0000104013.71118.53

Abstract

Objectives—Based on previous observations on apolipoprotein(a), apo(a), in human unstable carotid plaques, we explored whether in the inflammatory environment of human atheroma, proteolytic events affect other hepatic and topically generated proteins in relation to the issue of plaque stability.
Methods and Results—Forty unstable and 24 stable plaques from endarterectomy segments of affected human carotid arteries were extracted with buffered saline (PBS) and then 6 mol/L guanidine-hydrochloride (GdHCl) to identify loosely and tightly bound products, respectively. The extracts were studied before and after ultracentrifugation at d 1.21 g/mL. In the extracts, the concentrations of interleukin (IL)-6, -8, and -18 were significantly higher in the unstable plaques and correlated to those of MMP-2 and MMP-9. By Western blots, both apoB and apo(a) were highly fragmented and mostly present in the d 1.21 bottom that also contained fragments of apoE (10 and 22 kDa), decorin, biglycan, and versican. Fragmentation was higher in the unstable plaques. In baseline plasmas, concentrations of lipids, lipoproteins, and ILs did not differ between patients with unstable and stable plaques.
Conclusions—In unstable and to a lesser extent in stable plaques, there is a proinflammatory and proteolytic microenvironment with the generation of fragments with potential pathobiological significance that requires investigation.

Item Type:Article
ID Code:853
Status:Published
Refereed:Yes
Uncontrolled Keywords:Stable and unstable carotid plaques, apolipoproteins, interleukins, metalloproteinases, proteoglycans
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Lippincott Williams & Wilkins
eISSN:1524-4636
Copyright Holders:© American Heart Association
Deposited On:18 Aug 2009 10:03

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