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Functionalized multiwalled carbon nanotubes as ultrasound contrast agents

Delogu, Lucia Gemma and Vidili, Gianpaolo and Venturelli, Enrica and Ménard Moyon, Cécilia and Zoroddu, Maria Antonietta and Pilo, Giovanni Antonio and Nicolussi, Paola and Ligios, Ciriaco and Bedognetti, Davide and Sgarrella, Francesco and Manetti, Roberto and Bianco, Alberto (2012) Functionalized multiwalled carbon nanotubes as ultrasound contrast agents. Proceedings of The National Academy of Sciences, Vol. 109 (41), p. 16612-16617. eISSN 1091-6490. Article.

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DOI: 10.1073/pnas.1208312109

Abstract

Ultrasonography is a fundamental diagnostic imaging tool in everyday clinical practice. Here, we are unique in describing the use of functionalized multiwalled carbon nanotubes (MWCNTs) as hyperechogenic material, suggesting their potential application as ultrasound contrast agents. Initially, we carried out a thorough investigation to assess the echogenic property of the nanotubes in vitro. We demonstrated their long-lasting ultrasound contrast properties. We also showed that ultrasound signal of functionalized MWCNTs is higher than graphene oxide, pristine MWCNTs, and functionalized single-walled CNTs. Qualitatively, the ultrasound signal of CNTs was equal to that of sulfur hexafluoride (SonoVue), a commercially available contrast agent. Then, we found that MWCNTs were highly echogenic in liver and heart through ex vivo experiments using pig as an animal model. In contrast to the majority of ultrasound contrast agents, we observed in a phantom bladder that the tubes can be visualized within a wide variety of frequencies (i.e., 5.5–10 MHz) and 12.5 MHz using tissue harmonic imaging modality. Finally, we demonstrated in vivo in the pig bladder that MWCNTs can be observed at low frequencies, which are appropriate for abdominal organs. Importantly, we did not report any toxicity of CNTs after 7 d from the injection by animal autopsy, organ histology and immunostaining, blood count, and chemical profile. Our results reveal the enormous potential of CNTs as ultrasound contrast agents, giving support for their future applications as theranostic nanoparticles, combining diagnostic and therapeutic modalities.

Item Type:Article
ID Code:8462
Status:Published
Refereed:Yes
Uncontrolled Keywords:Nanomaterials, biocompatibility, nanomedicine, nanobiotechnology, echography
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 06 - Scienze mediche > MED/09 Medicina interna
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:The National Academy of Sciences of the USA
eISSN:1091-6490
Additional Information:Depositato per gentile concessione dell'Editore. È vietato duplicare e postare il PDF. È consentito l'uso a solo scopo di studio e di ricerca.
Deposited On:11 Dec 2012 11:37

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