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Analisi proteomica e funzionale del secreto di Trichomonas vaginalis

Cocco, Anna Rita (2013) Analisi proteomica e funzionale del secreto di Trichomonas vaginalis. Doctoral Thesis.

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Abstract

Trichomonas vaginalis is the causative agent of trichomoniasis, one of the most common sexually transmitted diseases in humans, recently associated with severe complications such as a significantly increased relative risk of developing cervical and prostate cancer. The mechanism by which T. vaginalis exerts the pathogenesis is only partly characterized. It was demonstrated that several secreted molecules play a central role, but proteins implicated are never directly observed. It was observed that lysis is dependent on pH, with a peak of activity at pH 5.8, lost when pH is raised over 6.5.
In our work, we optimised a protocol to obtain secreted proteins suitable for different proteomic approaches such as 1D and 2D electrophoresis. Our method allowed to identify in supernatants 114 and 202 proteins with heterogeneous functions obtained at pH 5.8 and 7.0, respectively.
Through bioinformatic analysis and electron microscopy, we shown that T. vaginalis secretes proteins via exosomes, a vesicular mediated mechanism that was not even reported in T. vaginalis.
We observed several differences between secretomes at pH 5.8 and 7.0, in particular for classes of proteases.
We identified an ortholog for human cytokine macrophage migration inhibitory factor that can be involved in the correlation with prostate cancer.
The database constructed by this work can be a start point for studies in pathogenesis, drug discovery and development of vaccination strategies for the control of trichomoniasis.

Item Type:Doctoral Thesis
ID Code:8434
Contributors:Fiori, Pier Luigi
Publisher:Università degli studi di Sassari
Uncontrolled Keywords:Trichomonas vaginalis, secretoma, patogenicità microbica
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Cicli, scuole e corsi:Ciclo 25 > Scuole > Scienze biomolecolari e biotecnologiche > Microbiologia molecolare e clinica
Deposited On:28 Mar 2013 12:49

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