Ledda, Sergio and Bogliolo, Luisa and Leoni, Giovanni Giuseppe and Naitana, Salvatore (1999) Follicular size affects the meiotic competence of in vitro matured prepubertal and adult oocytes in sheep. Reproduction Nutrition Development, Vol. 39 (4), p. 503-508. eISSN 1297-9708. Article.
Non-atretic follicles dissected from prepubertal and adult ovaries were allocated in three groups: a) < 1 mm; b) 1-2 mm; c) > 2 mm. After 24 h of maturation, a lower percentage of adult oocytes from group a (P < 0.01) reached metaphase II than those from groups b and c (70.4 versus 89.5 and 95.5). Prepubertal oocytes showed similar results (P < 0.01; 27.2 versus 79.8 and 81.8). There was a significant difference (P < 0.01) in meiotic progression between prepubertal and adult oocytes of < 1 mm follicles. The diameter of prepubertal oocytes derived from group a was significantly lower (P < 0.01) compared to groups b and c (138.1 versus 142.1 and 145.6); adult oocytes showed similar results (P < 0.01; 142.2 versus 157.2 and 158.1 Oocytes with the same diameter derived from different follicles of prepubertal and adult ovaries showed similar meiotic progression rates.
I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore
Repository Staff Only: item control page