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Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts

Ledda, Sergio and Bogliolo, Luisa and Leoni, Giovanni Giuseppe and Loi, Pasqualino and Cappai, Pietro and Naitana, Salvatore (1995) Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. Reproduction Nutrition Development, Vol. 35 (6), p. 629-637. eISSN 1297-9708. Article.

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DOI: 10.1051/rnd:19950603


This study compared the effect of using either CZB or TCM 199 media on both the development of 1-2 cell ovine embryos from superovulated ewes to the blastocyst stage (Experiment 1), and the hatching process of ovine blastocysts developed in vitro (Experiment 2). For the first 5 d, the CZB medium showed higher rates of embryo development than the TCM 199 medium (p < 0.001). The embryos reaching the > 16 cell stage were 79 vs 52% and 74 vs 20% with or without an oviductal monolayer, respectively, and those reaching the blastocyst stage were 71 vs 46% and 46 vs 13% with or without cells. The CZB medium was less able to support the hatching process of the blastocysts obtained in the first experiment than was the TCM-199 medium + 10% FCS (fetal calf serum) with cells (31 vs 92%; p < 0.001) or without cells (13 vs 66%; p < 0.001). No blastocysts completely escaped from the zona pellucida (ZP) in the CZB medium compared with 80 and 61 % in the TCM 199 medium with or without cells, respectively. In Experiment 3, 47% of the blastocysts migrated through the artificial opening of the ZP and hatched completely. After 24 h of culture in the CZB medium, however, they showed blastocoelic cavity breakdown. During the preliminary cleavages, the ovine embryos developed better in CZB medium than in TCM 199, but the latter was more efficient in promoting the hatching process of the blastocysts.

Les effets d’un milieu de culture, CZB, et d’un milieu de culture, TCM 199 + 10% de sérum de veau foetal, ont été observés sur le développement in vitro d’embryons d’ovins du stade 1-2 cellules au stade blastocyste (expérience 1) et dans le processus d’éclosion des blastocystes produits lors de l’expérience précédente (expérience 2). Le milieu CZB exerce un effet positif sur le développement embryonnaire par rapport au milieu TCM 199 après culture jusqu au stade > 16 cellules (79 vs 52 % et 74 vs 20%) ou jusqu’au stade blastocyste (71 vs 46% et 46 vs 13%) en présence de cellules et en l’absence de cellules tubaires respectivement. Le processus d’éclosion est plus faible dans le milieu CZB que dans le milieu TCM 199 soit en présence (31 vs 92%) soit en l’absence (13 vs 66%) de cellules tubaires respectivement. Dans le CZB (expérience 3), 47% des blastocystes éclosent par une fente artificielle dans la zone pellucide, mais, après 24 h de culture, la cavité blastocoelique se dégonfle. Ces résultats montrent que le milieu CZB est plus indiqué pour le développement précoce d’embryons ovins alors que le milieu TCM 199 est plus efficace au moment de la phase d’éclosion.

Item Type:Article
ID Code:8416
Uncontrolled Keywords:Culture media, ovine embryo development, hatching
Milieu de culture, développement embryonnaire, ovins, éclosion
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:EDP Sciences
Copyright Holders:© Elsevier/INRA
Publisher Policy:Depositato per gentile concessione dell'Editore
Deposited On:03 Dec 2012 16:20

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