Lamon, Sonia (2013) Prevalence, distribution and pathogenic profile of Vibrio parahaemolitycus and Vibrio spp. core in shellfish (Mytilus galloprovincialis and Ruditapes decussatus) collected in Sardinia (Italy): molecular fingerprinting using three pcr- based methods: BOX-pcr, REP-pcr and ERIC- pcr. Doctoral Thesis.
The aim of our study was to investigate the prevalence and concentration of Vibrio spp. core (V.s.) and V. parahaemolyticus (V.p.) in Mitylus galloprovincialis (M.g.) and Ruditapes decussatus (R.d.), using phenotypic and PCR methods. Detection of V.s. core have been carried out in samples (before and after depuration) of shellfish harvested in three areas of Sardinia (Italy), from April 2011 to May 2012. The prevalence of V.s. was 72.3% and 76.7%, and 90.0% and 78.5%, respectively in M.g and R.d., before and post depuration. A selection of the strains were submitted to PCR for species identification of V.s.. A prevalence of 2%, 0.40%, and 24 % of V. cholera, V. vulnificus and V.p., were respectively showed. The prevalence of V. p. was higher in R.d. (61%) than in M.g. (23%). Only 2/114 strains (1.8%) of. V.p. were positive for tdh+/trh- factors, and the same for tdh-/trh+, while none was tdh+/ trh+. V. p. strains were submitted to three fingerprinting PCR-based methods (ERIC-, REP- and BOX-PCR). Two clusters were obtained using all the techniques. Most of the strains were grouped in one of these, especially those isolated from clams in autumn, from the same area. Our findings confirm the potential human health risk associated with the presence of pathogenic V.s. in shellfish, and that vibrios shows a different feedback to the depuration processes. Then specific measures are needed to prevent the presence of naturally occurring bacteria in the molluscs.
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