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Molecular characterization of enterotoxigenic and borderline oxacillin resistant Staphylococcus strains from ovine milk

Perillo, Jusy and Ceccarelli, Daniela and Spagnoletti, Matteo and Lollai, Stefano and Cappuccinelli, Pietro Antonio and Colombo, Mauro Maria (2012) Molecular characterization of enterotoxigenic and borderline oxacillin resistant Staphylococcus strains from ovine milk. Food Microbiology, Vol. 32 (2), p. 265-273. ISSN 0740-0020. eISSN 1095-9998. Article.

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DOI: 10.1016/j.fm.2012.06.009


Staphylococcal enterotoxins (SEs) produced by Staphylococcus spp. are superantigens responsible for food-poisoning and are associated to mobile genetic elements such as Staphylococcus aureus pathogenicity islands (SaPI). The presence of 13 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sel, sek, seq, and tst) was tested in 15 S. aureus and 24 coagulase-negative Staphylococcus (CNS) multi-resistant strains isolated from ovine milk in Sardinia. All CNS isolates were enterotoxin-negative, whereas co-presence of sec, sel and tst was observed in most of the S. aureus strains. One isolate of S. aureus was characterized by tst alone. A multiplex PCR assay aimed at discriminating between the integrase genes of pathogenicity islands SaPI2, SaPIbov1, and SaPIMW2 was developed. We demonstrated that strains harboring sec, sel and tst were associated with SaPIbov1, whereas the strain positive for tst was associated with SaPI2. Borderline oxacillin resistant S. aureus strains were also detected. RAPD analysis of the Staphylococcus strains showed that clonal relationships were correlated with pathogenic profiles.

Item Type:Article
ID Code:8212
Uncontrolled Keywords:Staphylococcus aureus, CNS, ovine milk, enterotoxins, SaPI, genotyping, BORSA
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Deposited On:23 Nov 2012 13:32

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