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Recovery of Fusarium oxysporum Fo47 mutants affected in their biocontrol activity after transposition of the Fot1 element

Trouvelot, Sophie and Olivain, Chantal and Recorbet, Ghislaine and Migheli, Quirico and Alabouvette, Claude (2002) Recovery of Fusarium oxysporum Fo47 mutants affected in their biocontrol activity after transposition of the Fot1 element. Phytopathology, Vol. 92 (9), p. 936-945. ISSN 0031-949X. Article.

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DOI: 10.1094/PHYTO.2002.92.9.936

Abstract

To investigate the biocontrol mechanisms by which the antagonistic Fusarium oxysporum strain Fo47 is active against Fusarium wilt, a Fot1 transposon-mediated insertional mutagenesis approach was adopted to generate mutants affected in their antagonistic activity. Ninety strains in which an active Fot1 copy had transposed were identified with a phenotypic assay for excision and tested for their biocontrol activity against F. oxysporum f. sp. lini on flax in greenhouse experiments. Sixteen strains were affected in their capacity to protect flax plants, either positively (more antagonistic than Fo47) or negatively (less antagonistic). The molecular characterization of these mutants confirms the excision of Fot1 and its reinsertion in most of the cases. Moreover, we demonstrate that other transposable elements such as Fot2, impala, and Hop have no transposition activity in the mutant genomes. The phenotypic characterization of these mutants shows that they are affected neither in their in vitro growth habit nor in their competitiveness in soil compared with wild-type strain Fo47. These results show that mutants are not impaired in their saprophytic phase and suggest that the altered biocontrol phenotype should likely be expressed during the interaction with the host plant.

Item Type:Article
ID Code:82
Status:Published
Refereed:Yes
Uncontrolled Keywords:Linum usitatissimum, plant pathogenic fungi, Fusarium oxysporum, biological control, mutants, genetic engineering, insertional mutagenesis, gene transfer, transgenic plants, genetic variation, strain differences, pathotypes, disease control; plant Disease
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:The American Phytopathological Society
ISSN:0031-949X
Deposited On:18 Aug 2009 10:01

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