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Apricot melanoidins prevent oxidative endothelial cell death by counteracting mitochondrial oxidation and membrane depolarization

Cossu, Annalisa and Posadino, Anna Maria and Giordo, Roberta and Emanueli, Costanza and Sanguinetti, Anna Maria and Piscopo, Amalia and Poiana, Marco and Capobianco, Giampiero and Piga, Antonio <1965- > and Pintus, Gianfranco (2012) Apricot melanoidins prevent oxidative endothelial cell death by counteracting mitochondrial oxidation and membrane depolarization. PLoS One, Vol. 7 (11), e48817. eISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0048817

Abstract

The cardiovascular benefits associated with diets rich in fruit and vegetables are thought to be due to phytochemicals contained in fresh plant material. However, whether processed plant foods provide the same benefits as unprocessed ones is an open question. Melanoidins from heat-processed apricots were isolated and their presence confirmed by colorimetric analysis and browning index. Oxidative injury of endothelial cells (ECs) is the key step for the onset and progression of cardiovascular diseases (CVD), therefore the potential protective effect of apricot melanoidins on hydrogen peroxide-induced oxidative mitochondrial damage and cell death was explored in human ECs. The redox state of cytoplasmic and mitochondrial compartments was detected by using the redox-sensitive, fluorescent protein (roGFP), while the mitochondrial membrane potential (MMP) was assessed with the fluorescent dye, JC-1. ECs exposure to hydrogen peroxide, dose-dependently induced mitochondrial and cytoplasmic oxidation. Additionally detected hydrogen peroxide-induced phenomena were MMP dissipation and ECs death. Pretreatment of ECs with apricot melanoidins, significantly counteracted and ultimately abolished hydrogen peroxide-induced intracellular oxidation, mitochondrial depolarization and cell death. In this regard, our current results clearly indicate that melanoidins derived from heat-processed apricots, protect human ECs against oxidative stress.

Item Type:Article
ID Code:8154
Status:Published
Refereed:Yes
Uncontrolled Keywords:Apricot melanoidins, cardiovascular diseases (CVD), human endothelial cells (ECs)
Subjects:Area 06 - Scienze mediche > MED/40 Ginecologia e ostetricia
Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 07 - Scienze agrarie e veterinarie > AGR/15 Scienze e tecnologie alimentari
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Chirurgiche, Microchirurgiche e Mediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Public Library of Science
eISSN:1932-6203
Copyright Holders:© 2012 Cossu et al.
Deposited On:14 Nov 2012 09:39

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