titoli, abstracts, parole chiave >>>
Transposition of the miniature inverted-repeat transposable element mimp1 in the wheat pathogen Fusarium culmorum

Spanu, Francesca and Pasquali, Matias and Scherm, Barbara and Balmas, Virgilio and Marcello, Angela and Ortu, Giuseppe and Dufresne, Marie Thérèse and Hoffmann, Lucien and Daboussi, Marie-Josée and Migheli, Quirico (2012) Transposition of the miniature inverted-repeat transposable element mimp1 in the wheat pathogen Fusarium culmorum. Molecular Plant Pathology, Vol. 13 (9), p. 1149-1155. eISSN 1364-3703. Article.

Full text not available from this repository.

DOI: 10.1111/J.1364-3703.2012.00823.X


High-throughput methods are needed for functional genomics analysis in Fusarium culmorum, the cause of crown and foot rot on wheat and a type B trichothecene producer. Our aim was to develop and test the efficacy of a double-component system based on the ability of the impala transposase to transactivate the miniature inverted-repeat transposable element mimp1 of Fusarium oxysporum. We report, for the first time, the application of a tagging system based on a heterologous transposon and of splinkerette-polymerase chain reaction to identify mimp1 flanking regions in the filamentous fungus F. culmorum. Similar to previous observations in Fusarium graminearum, mimp1 transposes in F. culmorum by a cut-and-paste mechanism into TA dinucleotides, which are duplicated on insertion. mimp1 was reinserted in open reading frames in 16.4% (i.e. 10 of 61) of the strains analysed, probably spanning throughout the entire genome of F. culmorum. The effectiveness of the mimp1/impala double-component system for gene tagging in F. culmorum was confirmed phenotypically for a putative aurofusarin gene. This system also allowed the identification of two genes putatively involved in oxidative stress-coping capabilities in F. culmorum, as well as a sequence specific to this fungus, thus suggesting the valuable exploratory role of this tool.

Item Type:Article
ID Code:8127
Uncontrolled Keywords:Mutagenesis, Fusarium, transposon tagging, DNA transposons
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
Publisher:Wiley / Blackwell
Copyright Holders:© 2012 the authors, Molecular plant pathology © 2012 BSPP and Blackwell Publishing ltd
Deposited On:09 Nov 2012 12:26

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page