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Allestimento e trial clinico di un vaccino a DNA per il trattamento della leishmaniosi canina

Piras, Ilaria Michela (2013) Allestimento e trial clinico di un vaccino a DNA per il trattamento della leishmaniosi canina. Doctoral Thesis.

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Abstract

This study aimed to evaluate the effect of a DNA vaccine based on two Leishmania antigens (Cpb1, PO) in leishmaniotic dogs. Twelve leishmaniotic dogs from a leishmaniasis-endemic area (Naples, Italy) received three consecutive injections of DNA vaccine at 15-days intervals. Another group of five leishmaniotic dogs received the same amount of pVAX-1 without the coding sequences of Leishmania antigens. Leishmania DNA load, INFγ, IL-4 mRNA expression levels and clinical parameters were tested before and after the therapy, every 3 months for a period of 12 months.
Analysis of the data in the vaccinated dogs showed: i) a decrease Leishmania DNA load in lymph node samples, ii) an increase of INFγ and IL-levels in PBMC samples. All vaccinated dogs also showed an improvement in the clinical symptoms. Our results show that the vaccine developed in this study may represent a useful tool in the treatment of leishmaniotic dogs. However, since the duration of positive effects is limited in time, further trials are needed to evaluate the effectiveness of immunotherapy alone or in association with conventional therapy.

Item Type:Doctoral Thesis
ID Code:8095
Contributors:Pittau, Marco
Publisher:Università degli studi di Sassari
Uncontrolled Keywords:Leishmania infantum, vaccino a DNA, immunoterapia
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/05 Malattie infettive degli animali domestici
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Cicli, scuole e corsi:Ciclo 25 > Scuole > Scienze biomolecolari e biotecnologiche > Microbiologia molecolare e clinica
Deposited On:03 Apr 2013 16:25

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