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The Use of polyclonal activators in the production of murine monoclonal and polyclonal antibodies

Fiori, Pier Luigi and Rappelli, Paola (1991) The Use of polyclonal activators in the production of murine monoclonal and polyclonal antibodies. Journal of immunological methods, Vol. 139 (2), p. 181-190. eISSN 1872-7905. Article.

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Abstract

We propose a new immunization method to stimulate a strong immune response against weak or diluted antigens. This technique is based on stimulation with polyclonal activators before exposure to the antigens. We also discuss the efficiency of various types of mitogen with particular regard to their capacity to produce monoclonal antibodies and serum antibodies. A specific immune response against soluble antigens is increased by pretreating mice with PPD. This preactivation permitted us to obtain monoclonal antibodies against weak antigens in a few days. No monoclonal antibodies were obtained by inoculating weak antigens or the activators by themselves.

Item Type:Article
ID Code:7920
Status:Published
Refereed:Yes
Uncontrolled Keywords:Polyclonal activators, immunization method, monoclonal antibodies
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier
eISSN:1872-7905
Deposited On:31 Aug 2012 09:56

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