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Specific and common epitopes in mating pheromones of Euplotes raikovi revealed by monoclonal antibodies

Fiori, Pier Luigi and Miceli, Cristina and Raffioni, Simona and Vallesi, Adriana (1990) Specific and common epitopes in mating pheromones of Euplotes raikovi revealed by monoclonal antibodies. Journal of protozoology, Vol. 37 (3), p. 187-190. ISSN 0022-3921. Article.

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Abstract

Polypeptide mating pheromones Er-1 and Er-2, purified from the supernatant of Euplotes raikovi cultures of mating type I and mating type II, respectively, were used to immunize mice and obtain monoclonal antibodies. Five hybridoma clones producing antibodies specific to the mating pheromones were selected. They were analyzed for immunospecificity by immunoperoxidase assay, immunoblotting, and for their efficacy in inhibition of mating pheromone activity. Monoclonal antibodies from two hybridoma clones recognized only the mating pheromone used as antigen: those from the other three clones reacted, to comparable extents, with both mating pheromones. On the basis of these results it was assumed that two immunogenic sites exist in Er-1 and Er-2, one specific and the other common to both mating pheromones.

Item Type:Article
ID Code:7919
Status:Published
Refereed:Yes
Uncontrolled Keywords:Euplotes raikovi, Polypeptide mating pheromones Er-1, Er-2, monoclonal antibodies
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Society of Protozoologists
ISSN:0022-3921
Deposited On:31 Aug 2012 09:36

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